Neuropilin-2 Protein, Human, Recombinant (hFc)

Neuropilin-2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 120.7 kDa and the accession number is O60462-3.

Neuropilin-2 Protein, Human, Recombinant (hFc)

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 88,500
100 μg	約5 days	￥ 142,500
200 μg	約5 days	￥ 228,500
500 μg	約5 days	￥ 427,000

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生物学的情報	1.Measured by its binding ability in a functional ELISA. 2. Immobilized human VEGFC-His at 10μg/mL (100μL/well) can bind human NRP2-Fc , the EC50 of human NRP2-Fc is 0.1-1μg/mL.
説明	Neuropilin-2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 120.7 kDa and the accession number is O60462-3.

Species	Human
Expression Host	HEK293 Cells
Tag	C-hFc
Accession Number	O60462-3
別名	Neuropilin-2, NPN2, neuropilin 2, PRO2714, VEGF165R2, NP2
Construction	The Human NRP2 (NP_003863.2)(Met1-Tyr855) was expressed with the Fc region of Human IgG1 at the C-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	120.7 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Neuropilin-2 (NRP-2) which is related to NRP-1, is a type I? transmembrane glycoprotein and has the structure characteristic with five main extracellular domains: two complement binding (CUB) domains, two coagulation factor V/VIII homology domains, and a MAM (meprin, tyrosine phosphatase domain) region. NRP-2 is a receptor capable of binding two disparate ligands, classⅢ semaphorins (SEMA) and vascular endothelial growth factors (VEGF), and thus regulates two diverse systems by activating cellular signaling pathways via interacting with other cell surface receptors such as VEGF receptors and plexins. NRP-2 is well known for its role in facilitating axonal guidance during the development of the neuronal system, and additionally, it is also expressed in vascular endothelial cells and lymphatic endothelium where it affects proliferation, migration, angiogenesis, as well as the formation of small lymphatic vessels and capillaries. A recent study has identified NRP-2 as a polysialylation protein expressed in human dendritic cells and modulates DC-T Cell Interactions. Nearly all tumor cells express neuropilins and NRP-2 is predominantly expressed in neuronal tumors and melanomas. Furthermore, it is suggested that as the specific ligand for NRP-2, SEMA 3F inhibits tumor angiogenesis, and metastasis.

参考文献

1. Chen H. et al., 1997, Neuron. 19: 547-59. 2. Bielenberg DR. et al., 2006, Exp Cell Res. 312: 584-93. 3. Yuan L. et al., 2002, Development. 129: 4797-806. 4. Benoit F. et al., 2006, Blood. 108: 1243-50. 5. Sabrina C. et al., 2007, J Biol Chem. 282: 30346-56. 6. Gray MJ. et al., 2008, J Natl Cancer Inst. 100: 109-20.

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Neuropilin-2 Protein, Human, Recombinant (hFc)

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