NGF Protein, Human, Recombinant

NGF Protein, Human, Recombinant is expressed in CHO mammalian cells. The predicted molecular weight is 13.2 kDa and the accession number is P01138.

NGF Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 36,000

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生物学的情報	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-2 ng/ml.
説明	NGF Protein, Human, Recombinant is expressed in CHO mammalian cells. The predicted molecular weight is 13.2 kDa and the accession number is P01138.

Species	Human
Expression Host	CHO Cells
Tag	Tag Free
Accession Number	P01138
別名	Beta-NGF, β-NGF, NGFB, nerve growth factor (β polypeptide), nerve growth factor (beta polypeptide), HSAN5
Construction	mature form of Human β-NGF (NP_002497.2) (Ser 122-Arg 239)
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	13.2 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 40 mM His, 40 mM Arg, 150 mM NaCl, pH 5. 5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth-stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein that homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. Also, previous studies indicated that NGF may also have an important role in the regulation of the immune system.

参考文献

1. Castellanos MR, et al. (2003) Evaluation of the neurorestorative effects of the murine beta-nerve growth factor infusions in old rat with cognitive deficit. Biochem Biophys Res Commun. 312(4): 867-72. 2. Wang TH, et al. (2008) Effects of pcDNA3-beta-NGF gene-modified BMSC on the rat model of Parkinson's disease. J Mol Neurosci. 35(2): 161-9. 3. Perrard MH, et al. (2009) Redundancy of the effect of TGFbeta1 and beta-NGF on the second meiotic division of rat spermatocytes. Microsc Res Tech. 72(8): 596-602.

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NGF Protein, Human, Recombinant

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