NGF Protein, Mouse, Recombinant is expressed in CHO mammalian cells. The predicted molecular weight is 13.5 kDa and the accession number is P01139.
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
20 μg | 約5 days | ¥ 38,000 |
100 μg | 約5 days | ¥ 106,000 |
1 mg | 約5 days | ¥ 688,500 |
説明 | NGF Protein, Mouse, Recombinant is expressed in CHO mammalian cells. The predicted molecular weight is 13.5 kDa and the accession number is P01139. |
Species | Mouse |
Expression Host | CHO Cells |
Tag | Tag Free |
Accession Number | P01139 |
別名 | nerve growth factor (β polypeptide), Ngfb, nerve growth factor (beta polypeptide) |
Construction | The mature form of Mouse NGF (NP_001106168.1) (Ser 122-Gly 241) was expressed, with an initial Met at the C-terminus. |
Protein Purity | > 96 % as determined by SDS-PAGE |
分子量 | 13.5 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 150 mM NaCl, 50 mM NaAc, pH 5. 5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth-stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein that homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. Also, previous studies indicated that NGF may also have an important role in the regulation of the immune system. |
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Please read the User Guide of Recombinant Proteins for more specific information.
NGF Protein, Mouse, Recombinant b polypeptide nerve growth factor beta polypeptide nerve growth factor (β polypeptide) β polypeptide Ngfb nerve growth factor (beta polypeptide) recombinant recombinant-proteins proteins protein