NKG2D/CD314 Protein, Human, Recombinant (aa 78-216, His)

NKG2D/CD314 Protein, Human, Recombinant (aa 78-216, His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 18.4 kDa and the accession number is P26718-1.

NKG2D/CD314 Protein, Human, Recombinant (aa 78-216, His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 88,500
200 μg	約5 days	￥ 156,500
500 μg	約5 days	￥ 330,500

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生物学的情報	1. Immobilized human His-NKG2D (78-216) at 2 μg/ml (100 μl/well) can bind human ULBP1-hFc , The EC50 of human MICB-hFc is 8-50 ng/mL 2. Immobilized human His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human ULBP1-hFc , The EC50 of human MICB-hFc is 15-60 ng/mL.
説明	NKG2D/CD314 Protein, Human, Recombinant (aa 78-216, His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 18.4 kDa and the accession number is P26718-1.

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	His
Accession Number	P26718-1
別名	KLR, killer cell lectin-like receptor subfamily K, member 1, D12S2489E, CD314, NKG2-D, NKG2D
Construction	The Human NKG2D (P26718-1) (Phe78-Val216) was fused with a polyhistide tag at the N-terminus.
Protein Purity	≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量	18.4 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 300 mM NaCl, 10% glycerol, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	KLRK1 (Killer Cell Lectin Like Receptor K1) is a Protein Coding gene. NKG2D, also known as CD314, is an immune receptor that consists of two disulfide-linked type II transmembrane proteins with short intracellular proteins incapable to transduce signals. To transduce signals, NKG2D needs adaptor proteins and it uses two adaptor proteins, DAP10 and DAP12. These two adaptor proteins associate as homodimers to NKG2D- therefore the entire receptor complex appears as a hexamer. NKG2D can send co-stimulatory signals to activate CD8 T cells. NKG2D also plays an important role in viral control. Cellular stress can induce ligands for NKG2D which results in the cell susceptible to NK cell-mediated lysis.

参考文献

1. Houchins J, et al. (1991) DNA sequence analysis of NKG2, a family of related cDNA clones encoding type II integral membrane proteins on human natural killer cells. J Exp Med. 173: 1017-102. 2. Bauer S, et al. (1999) Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science. 285(5428):727-9. 3. Zafirova B, et al. (2011) Regulation of immune cell function and differentiation by the NKG2D receptor. Cell Mol Life Sci. 68(21):3519-29.

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NKG2D/CD314 Protein, Human, Recombinant (aa 78-216, His)

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