Nucleoside phosphorylase/PNP Protein, Human, Recombinant (His)

Purine nucleoside phosphorylase (PNP) is a purine-metabolizing enzyme that catalyzes the reversible phosphorolysis of 6-oxypurine (deoxy)nucleosides to their respective bases and (deoxy)ribose-1-phosphate. It is a key enzyme in the purine salvage pathway of mammalian cells. Purine nucleoside phosphorylase is a transferase that catalyzes the addition of phosphate and removal of a purine base from guanosine and similar nucleosides.PNP defects result in metabolic abnormalities and fatal T cell immunodeficiency. Purine nucleoside phosphorylase (PNP) is a target for leukemia, gout, and autoimmune disorders.

Nucleoside phosphorylase/PNP Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 88,500

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生物学的情報

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説明

Species	Human
Expression Host	E. coli
Tag	His
Accession Number	P00491
別名	PUNP, PRO1837, purine nucleoside phosphorylase, NP
Construction	A DNA sequence encoding the human PNP (P00491) (Met 1-Ser 289) was fused with a polyhistide tag at the C-terminus.
Protein Purity	> 97 % as determined by SDS-PAGE
分子量	33.5 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Supplied as sterile PBS, 25% glycerol, pH 7.5. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	Solution. It is shipped out with blue ice.
Research Background	Purine nucleoside phosphorylase (PNP) is a purine-metabolizing enzyme that catalyzes the reversible phosphorolysis of 6-oxypurine (deoxy)nucleosides to their respective bases and (deoxy)ribose-1-phosphate. It is a key enzyme in the purine salvage pathway of mammalian cells. Purine nucleoside phosphorylase is a transferase that catalyzes the addition of phosphate and removal of a purine base from guanosine and similar nucleosides.PNP defects result in metabolic abnormalities and fatal T cell immunodeficiency. Purine nucleoside phosphorylase (PNP) is a target for leukemia, gout, and autoimmune disorders.

参考文献

1. de Azevedo,W.F. et al., 2003, Biochem Biophys Res Commun. 312 (3): 767-72. 2. Canduri,F. et al., 2005, Acta Crystallogr D Biol Crystallogr. 61 (Pt 7): 856-62. 3. Perera,G.K. et al., 2005,Clin Exp Dermatol. 30 (1):27-9.

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Nucleoside phosphorylase/PNP Protein, Human, Recombinant (His)

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