Osteoactivin/GPNMB Protein, Human, Recombinant (hFc)

Osteoactivin/GPNMB Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 77.8 kDa and the accession number is Q14956-2.

Osteoactivin/GPNMB Protein, Human, Recombinant (hFc)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 138,000

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説明	Osteoactivin/GPNMB Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 77.8 kDa and the accession number is Q14956-2.

Species	Human
Expression Host	HEK293 Cells
Tag	C-hFc
Accession Number	Q14956-2
別名	glycoprotein (transmembrane) nmb, NMB, HGFIN, Osteoactivin
Construction	The Human GPNMB (Q14956-2) (Met1-Pro474) was expressed with the Fc region of Human IgG1 at the C-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	77.8 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	GPNMB belongs to the PMEL / NMB family, also known as Osteoactivin and Hematopoietic growth factor-inducible neurokinin 1 ( HGFIN ), is a transmembrane glycoprotein that is expressed in numerous cells, including osteoclasts, macrophages, dendritic cells, and tumor cells. It is suggested to influence osteoblast maturation, cell adhesion, and migration. GPNMB protein acts as a downstream mediator of BMP-2 effects on osteoblast differentiation and function. GPNMB participates in bone mineralization and functions as a negative regulator of inflammation in macrophages. Osteoactivin is expressed at high levels in normal and inflammatory liver macrophages suggesting a significant role in acute liver injury. The early-phase upregulation of Osteoactivin expression in the tubular epithelium in response to renal injury might play a role in triggering renal interstitial fibrosis via activation of matrix metalloproteinase expression and collagen remodeling in rats. Osteoactivin is a protein that is expressed in aggressive human breast cancers and is capable of promoting breast cancer metastasis to bone.

参考文献

1. Pahl MV. et al., 2010, Clin J Am Soc Nephrol. 5(1): 56-61. 2. Abdelmagid SM. et al., 2008, Exp Cell Res. 314(13): 2334-51. 3. Haralanova-Ilieva B. et al., 2005, J Hepatol. 242(4): 565-72. 4. Abdelmagid SM. et al., 2007, J Cell Physiol. 210(1): 26-37. 5. Furochi H. et al., 2007, J Med Invest. 54(3-4): 248-54.

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Osteoactivin/GPNMB Protein, Human, Recombinant (hFc)

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