OX40/TNFRSF4 Protein, Human, Recombinant (aa 29-216, His & Avi), Biotinylated

Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation. OX40/TNFRSF4 Protein, Human, Recombinant (aa 29-216, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 23.2 kDa and the accession number is P43489.

OX40/TNFRSF4 Protein, Human, Recombinant (aa 29-216, His & Avi), Biotinylated

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 187,000
500 μg	約5 days	￥ 746,000
1 mg	約5 days	￥ 1,248,500

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説明

Species	Human
Expression Host	HEK293 Cells
Tag	C-His-Avi
Accession Number	P43489
別名	IMD16, TNFRSF4, CD134, ACT-135, TXGP1L, OX40 homologue, OX40, OX40L receptor, Ly-70
Construction	Leu29-Ala216
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	23.2 kDa (predicted). Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation.

参考文献

1. Al-Shamkhani A, et al. Affinity and Kinetics of the Interaction between Soluble Trimeric OX40 Ligand, a Member of the Tumor Necrosis Factor Superfamily, and Its Receptor OX40 on Activated T Cells[J]. Journal of Biological Chemistry, 1997, 272(8):5275-5282.

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OX40/TNFRSF4 Protein, Human, Recombinant (aa 29-216, His & Avi), Biotinylated

参考文献

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