p38 gamma/MAPK12 Protein, Human, Recombinant (His & GST)

p38 gamma/MAPK12 Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 69.8 kDa and the accession number is P53778.

p38 gamma/MAPK12 Protein, Human, Recombinant (His & GST)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 114,500

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説明	p38 gamma/MAPK12 Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 69.8 kDa and the accession number is P53778.

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	His, GST
Accession Number	P53778
別名	ERK3, SAPK3, PRKM12, ERK6, P38γ, P38GAMMA, mitogen-activated protein kinase 12, P38 γ/MAPK12, MAPK12, ERK-6, SAPK-3
Construction	The Human MAPK12 (P53778) (Met1-Leu367) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Protein Purity	≥ 90 % as determined by SDS-PAGE
分子量	69.8 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.0.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Research Background	ERK3, also known as MAPK12 and p38-gamma, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, and MAP kinase subfamily. ERK3 is highly expressed in skeletal muscle and heart. ERK3 is a serine/threonine kinase that acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 2 to 3 substrates each. MAPK12 is required for the normal kinetochore localization of PLK1, prevents chromosomal instability, and supports mitotic cell viability. MAPK12-signaling is also positively regulating the expansion of transient amplifying myogenic precursor cells during muscle growth and regeneration.

参考文献

1. Stiffler MA. et al., 2006, J Am Chem Soc. 128 (17): 5913-22. 2. Joneson T. et al., 1997, J Mol Med. 75 (8): 587-93. 3. Hou SW. et al., 2010, Cancer Res. 70 (7): 2901-10.

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p38 gamma/MAPK12 Protein, Human, Recombinant (His & GST)

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