P4HB Protein, Human, Recombinant (His)

P4HB Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 56.4 kDa and the accession number is A0A024R8S5.

P4HB Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 88,500
200 μg	約5 days	￥ 151,500
500 μg	約5 days	￥ 307,500

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生物学的情報	Measured by its ability to promote aggregation of insulin in the presence of DTT. The specific activity is > 7.5 A650/min/mg
説明	P4HB Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 56.4 kDa and the accession number is A0A024R8S5.

Species	Human
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	A0A024R8S5
別名	DSI, prolyl 4-hydroxylase, β polypeptide, PDIA1, PDI, GIT, ERBA2L, P4Hbeta, prolyl 4-hydroxylase, beta polypeptide, PO4DB, PO4HB, P4Hβ, PHDB, PROHB
Construction	Human PH4B (NP_000909.2) corresponding to amino acid (Met 1-Lys 505)
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	56.4 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Protein disulfide-isomerase, also known as Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55 and P4HB, is a peripheral membrane protein that belongs to the protein disulfide isomerase family. P4HB is highly abundant. In some cell types, it seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources. P4HB localizes near CD4-enriched regions on lymphoid cell surfaces. It is identified by mass spectrometry in melanosome fractions from stage I to stage IV. P4HB reduces and may activate fusogenic properties of HIV-1 gp12 surface protein, thereby enabling HIV-1 entry into the cell. P4HB catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, it seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. P4HB may therefore cause structural modifications of exofacial proteins. Inside the cell, it seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, P4HB functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). P4HB may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts as a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.

参考文献

1. Kivirikko KI, et al., 1989, FASEB J., 3 (5): 1609-17. 2. Pihlajaniemi T, et al.,1991, J Hepatol., 13, Suppl 3: S2. 3. Fenouillet E., et al., 2001, J. Infect. Dis. 183:744-752. 4. Gevaert K., et al., 2003, Nat. Biotechnol. 21:566-569. 5. Barbouche R., et al., 2003, J. Biol. Chem. 278:3131-3136.

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P4HB Protein, Human, Recombinant (His)

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