P-Selectin Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 81.4 kDa and the accession number is AAN06828.1.
生物学的情報 | Testing in progress |
説明 | P-Selectin Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 81.4 kDa and the accession number is AAN06828.1. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | His |
Accession Number | AAN06828.1 |
別名 | GMP140, CD62, CD62P, LECAM3, P-Selectin, GRMP, selectin P (granule membrane protein 140kDa, antigen CD62), PSEL, PADGEM |
Construction | The Human SELP (AAN06828.1) extracellular domain (Met 1-Ala 771) was fused with a polyhistidine tag at the C-terminus. |
Protein Purity |
≥ 90 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.
|
分子量 | 81.4 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. |
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Please read the User Guide of Recombinant Proteins for more specific information.
P-Selectin Protein, Human, Recombinant (His) LECAM 3 GMP140 selectin P LECAM-3 CD62 granule membrane protein 140kDa, antigen CD62 CD62P CD-62 LECAM3 P-Selectin GMP 140 CD 62 GRMP selectin P (granule membrane protein 140kDa, antigen CD62) GMP-140 PSEL PADGEM recombinant recombinant-proteins proteins protein