PCSK9 Protein, Human, Recombinant (His & Avi), Biotinylated

PCSK9 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 74.3 kDa and the accession number is Q8NBP7-1.

PCSK9 Protein, Human, Recombinant (His & Avi), Biotinylated

パッケージサイズ	在庫状況	単価(税別)
20 μg	在庫あり	￥ 67,000
100 μg	約5 days	￥ 236,500
200 μg	約5 days	￥ 404,000
500 μg	約5 days	￥ 817,000

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生物学的情報	Measured by its ability to bind recombinant LDLR-His in a functional ELISA.
説明	PCSK9 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 74.3 kDa and the accession number is Q8NBP7-1.

Species	Human
Expression Host	HEK293 Cells
Tag	His, Avi
Accession Number	Q8NBP7-1
別名	NARC-1, PC9, LDLCQ1, NARC1, FH3, proprotein convertase subtilisin/kexin type 9, HCHOLA3
Construction	The PCSK9 (NP_777596.2) (Met1-Gln692) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Protein Purity	> 95 % as determined by SDS-PAGE.
分子量	74.3 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.

参考文献

1. Sseidah, N.G. et al., 2003, Proc. Natl. Acad. Sci. USA. 100: 928-933. 2. Beyer, T.P. et al., 2007, J. Lipid. Res. 48: 1488-1498 3. Shan, L. et al., 2008, Biochem. Biophys. Res. Commun. 375: 69-73. 4. Benjannet, S. et al., 2005, J. Biol. Chem. 279: 48865-48875. 5. Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.

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PCSK9 Protein, Human, Recombinant (His & Avi), Biotinylated

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