PD-L1 Protein, Human, Recombinant (aa 19-238, His & Avi), Biotinylated

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1 Protein, Human, Recombinant (aa 19-238, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 28.1 kDa and the accession number is Q9NZQ7-1.

PD-L1 Protein, Human, Recombinant (aa 19-238, His & Avi), Biotinylated

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 187,000
500 μg	約5 days	￥ 746,000
1 mg	約5 days	￥ 1,248,500

バルクのお問い合わせ

説明

Species	Human
Expression Host	HEK293
Tag	C-His-Avi
Accession Number	Q9NZQ7-1
別名	PDL1, PD-L1B7 homolog 1, PD-L1, B7H1, PDCD1L1, B7-H1, CD274, B7-H, PDCD1LG1
Construction	Recombinant Biotinylated Human PD-L1/B7-H1 is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Phe19-Arg238.[Accession \|Q9NZQ7-1]
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	The protein has a predicted MW of 28.1 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.

Endotoxin	Less than 1EU per μg by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.

参考文献

1. Blank C , Mackensen A. Contribution of the PD-L1/PD-1 pathway to T-cell exhaustion: an update on implications for chronic infections and tumor evasion[J]. Cancer Immunology Immunotherapy, 2007, 56(5):739-745.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

PD-L1 Protein, Human, Recombinant (aa 19-238, His & Avi), Biotinylated

参考文献

Calculator

技術サポート

Keywords