PD-L1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 26.8 kDa and the accession number is Q9NZQ7-1.
パッケージサイズ | 在庫状況 | 単価(税別) |
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100 μg | 在庫あり | ¥ 73,500 |
200 μg | 約5 days | ¥ 130,500 |
500 μg | 約5 days | ¥ 275,500 |
生物学的情報 |
1. Immobilized Anti-PDL1(GEN)-IgG1 Antibody (Atezolizumab) at 2 μg/mL (100 μL/well) can bind Recombinant Human PD-L1/B7-H1/CD274 Protein (His Tag), the EC50 is 0.7-2.1 ng/mL. 2. Captured Anti-PD-L1 Mab (Human IgG1) on proA Chip can bind PD-L1 with an affinity constant of 1.381 nM as determined in a SPR assay (QC tested).
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説明 | PD-L1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 26.8 kDa and the accession number is Q9NZQ7-1. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | C-His |
Accession Number | Q9NZQ7-1 |
別名 | PDL1, B7-H, B7H1, PDCD1LG1, CD274 molecule, PD-L1, PDCD1L1, B7-H1 |
Construction | N-terminal segment (Met 1-Thr 239) of the extracellular domain of Human B7-H1 (NP_054862.1) |
Protein Purity |
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
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分子量 | 26.8 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Blockade: Blocking AntibodiImmune Checkpoint Blockade: PD-L1 / B7-H1 / CImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint Detection: IHC AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyPD-L1 / B7-H1 / CD274 Immune Checkpoint ProteTargeted Therapy |
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Please read the User Guide of Recombinant Proteins for more specific information.
PD-L1 Protein, Human, Recombinant (His) PDL 1 PDL1 B7-H B7H1 PDCD1LG1 CD274 molecule PD-L1 PDL-1 PDCD1L1 B7-H1 recombinant recombinant-proteins proteins protein