PD-L2 Protein, Human, Recombinant (His & Avi)

PD-1 ligand 2 (PD-L2) as a second ligand for PD-1 and compare the function and expression of PD-L1 and PD-L2. Engagement of PD-1 by PD-L2 dramatically inhibits T cell receptor (TCR)-mediated proliferation and cytokine production by CD4 T cells. At low antigen concentrations, PD-L2-PD-1 interactions inhibit strong B7-CD28 signals. PD-L2 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 25.3 kDa and the accession number is Q9BQ51.

PD-L2 Protein, Human, Recombinant (His & Avi)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 74,500
500 μg	約5 days	￥ 298,500
1 mg	約5 days	￥ 498,000

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説明

Species	Human
Expression Host	HEK293
Tag	C-His-Avi
Accession Number	Q9BQ51
別名	PDL2, Btdc, Butyrophilin B7-DC, PDCD1LG2, bA574F11.2, CD273, PDCD1 ligand 2, B7DC, PD-L2, PDCD1L2
Construction	Recombinant Human PD-L2/B7-DC Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Leu20-Thr220.[Accession \|Q9BQ51]
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	The protein has a predicted MW of 25.3 kDa. Due to glycosylation, the protein migrates to 40-52 kDa based on Tris-Bis PAGE result.

Endotoxin	Less than 1EU per μg by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	PD-1 ligand 2 (PD-L2) as a second ligand for PD-1 and compare the function and expression of PD-L1 and PD-L2. Engagement of PD-1 by PD-L2 dramatically inhibits T cell receptor (TCR)-mediated proliferation and cytokine production by CD4 T cells. At low antigen concentrations, PD-L2-PD-1 interactions inhibit strong B7-CD28 signals.

参考文献

1. Yearley JH, et al. PD-L2 Expression in Human Tumors: Relevance to Anti-PD-1 Therapy in Cancer. Clin Cancer Res. 2017;23(12):3158-3167. doi:10.1158/1078-0432.CCR-16-1761

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PD-L2 Protein, Human, Recombinant (His & Avi)

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