PD-L2 Protein, Human, Recombinant (His), Biotinylated

PD-L2 Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 24 kDa and the accession number is Q9BQ51-1.

PD-L2 Protein, Human, Recombinant (His), Biotinylated

パッケージサイズ	在庫状況	単価(税別)
20 μg	在庫あり	￥ 45,500
100 μg	約5 days	￥ 107,500
200 μg	約5 days	￥ 183,000
500 μg	約5 days	￥ 369,500

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生物学的情報	Measured by its binding ability in a functional ELISA. Immobilized Human PD-1 hFc at 2 μg/ml (100 μl/well) can bind Human PD-L2 His, Biotinylated, the EC50 of Human PD-L2 His, Biotinylated is 300-900 ng/mL.
説明	PD-L2 Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 24 kDa and the accession number is Q9BQ51-1.

Species	Human
Expression Host	HEK293
Tag	His
Accession Number	Q9BQ51-1
別名	B7DC, Btdc, B7-DC, bA574F11.2, PDL2, programmed cell death 1 ligand 2, PDCD1L2, PD-L2, CD273
Construction	A DNA sequence encoding the extracellular domain (Met1-Pro219) of human PD-L2 (NP_079515.2) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.
Protein Purity	> 95% as determined by SDS-PAGE.
分子量	24 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS,pH7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Programmed death ligand 2 (PD-L2), also referred to as B7-DC and CD273, is a member of the B7 family of proteins including B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS in B cells, INF-γ in monocytes, or LPS plus IFN-γ in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1, member of the CD28/CTLA4 family expressed on activated lymphoid cells, and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1/PD-1.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy

参考文献

1. Latchman Y,et al.(2001) PD-L2 is a second ligand for PD-1 and inhibits T cell activation. Nat Immunol. 2: 261-8. 2. Carreno BM,et al.(2005) Therapeutic opportunities in the B7/CD28 family of ligands and receptors. Curr Opin Pharmacol. 5(4): 424-30. 3. Radhakrishnan S,et al.(2007) B7-DC/PD-L2 cross-linking induces NF-kappaB-dependent protection of dendritic cells from cell death. J Immunol. 178(3): 1426-32.

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PD-L2 Protein, Human, Recombinant (His), Biotinylated

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