PDE2A Protein, Human, Recombinant (aa 215-900, His)

カタログ番号 TMPY-02081

別名: phosphodiesterase 2A, cGMP-stimulated, cGSPDE, PED2A4, PDE2A1, CGS-PDE

cGMP-dependent 3',5'-cyclic phosphodiesterase, also known as cyclic GMP-stimulated phosphodiesterase and PDE2A, is a peripheral membrane protein that belongs to the cyclic nucleotide phosphodiesterase family and PDE2 subfamily. Phosphodiesterases (PDEs) comprise a family of enzymes that regulate the levels of cyclic nucleotides, key second messengers that mediate a diverse array of functions. Phosphodiesterases (PDEs) modulate signaling by cyclic nucleotides in diverse processes such as cardiac contractility, platelet aggregation, lipolysis, glycogenolysis, and smooth muscle contraction. PDE2A is an evolutionarily conserved cGMP-stimulated cAMP and cGMP PDE. PDE2A contains two GAF domains. PDE2A is expressed in brain and to a lesser extent in heart, placenta, lung, skeletal muscle, kidney and pancreas. PDE2A is a cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. PDE2A is involved in the regulation of blood pressure and fluid homeostasis by the atrial natriuretic peptide (ANP), making PDE2-type enzymes important targets for drug discovery.

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PDE2A Protein, Human, Recombinant (aa 215-900, His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 138,000

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生物学的特性に関する説明

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Product Properties

参考文献

説明

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	C-His
Accession Number	O00408-1
別名	phosphodiesterase 2A, cGMP-stimulated, cGSPDE, PED2A4, PDE2A1, CGS-PDE
Construction	The amino acids (Glu 215-His 900) of Human PDE2A (O00408-1) was fused with a polyhistidine tag at the C-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	80.73 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 7.4, 10% gly. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	cGMP-dependent 3',5'-cyclic phosphodiesterase, also known as cyclic GMP-stimulated phosphodiesterase and PDE2A, is a peripheral membrane protein that belongs to the cyclic nucleotide phosphodiesterase family and PDE2 subfamily. Phosphodiesterases (PDEs) comprise a family of enzymes that regulate the levels of cyclic nucleotides, key second messengers that mediate a diverse array of functions. Phosphodiesterases (PDEs) modulate signaling by cyclic nucleotides in diverse processes such as cardiac contractility, platelet aggregation, lipolysis, glycogenolysis, and smooth muscle contraction. PDE2A is an evolutionarily conserved cGMP-stimulated cAMP and cGMP PDE. PDE2A contains two GAF domains. PDE2A is expressed in brain and to a lesser extent in heart, placenta, lung, skeletal muscle, kidney and pancreas. PDE2A is a cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. PDE2A is involved in the regulation of blood pressure and fluid homeostasis by the atrial natriuretic peptide (ANP), making PDE2-type enzymes important targets for drug discovery.

参考文献

1. Iffland,A. et al., 2005, Biochemistry. 44 (23):8312-25. 2. de Oliveira,S.K. et al., 2007,J Biol Chem. 282 (18):13656-63. 3. Stephenson,D.T. et al., 2009, J Histochem Cytochem. 57 (10):933-49. 4. Russwurm,C. et al., 2009, J Biol Chem. 284 (38):25782-90.

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Keywords

PDE2A Protein, Human, Recombinant (aa 215-900, His) phosphodiesterase 2A, cGMP-stimulated cGSPDE PED2A4 PDE2A1 CGS-PDE recombinant recombinant-proteins proteins protein