PDGF-AA Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 16 KDa and the accession number is P04085.
| 説明 |
PDGF-AA Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 16 KDa and the accession number is P04085. |
| Species |
Human
|
| Expression Host |
E. coli
|
| Tag |
Tag Free
|
| Accession Number |
P04085
|
| 別名 |
PDGFAA, PDGF-AA
|
| Amino Acid |
Ser87-Thr211
|
| Construction |
Ser87-Thr211
|
| Protein Purity |
Greater than 90% as determined by reducing SDS-PAGE. Greater than 90% as determined by SEC-HPLC.
|
| 分子量 |
16 KDa (reducing condition)
|
| Endotoxin |
< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
|
| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Glycine-HCl, 6% Sucrose, 4% Mannitol, 0.02% Tween 80, pH 3.0.
|
| Reconstitution |
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
|
| Stability & Storage |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
|
| Shipping |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
|
| Research Background |
Platelet-derived growth factor subunit A (PDGFA), belongs to the PDGF/VEGF growth factor family. PDGFA is a secreted protein, stored in platelet alpha-granules and released by platelets upon wounding. PDGFA is potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. It plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. PDGFA is required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis, normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. It plays an important role in wound healing; Signaling is modulated by the formation of heterodimers with PDGFB.
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