Platelet-derived growth factor-B (PDGFB) is necessary for normal cardiovascular development. The administration of PDGFB alone normalized tumor vasculature by increasing periendothelial coverage and vascular functionality. Interestingly, this effect exerted by PDGFB was also observed in the presence of DAPT. So PDGFB is able to improve tumor vascularity and allows the anticancer action of DAPT in the tumor. PDGFB Protein, Human, Recombinant (His) is expressed in yeast with His tag. The predicted molecular weight is 14.3 kDa and the accession number is P01127-2.
| 生物学的情報 |
Measured by its binding ability in a functional ELISA. Immobilized Human PDGF-B His at 2 μg/ml (100 μl/well) can bind Human PDGFRBHis & hFc, the EC50 of Human PDGFRBHis & hFc is 9.0-40.0 ng/mL.
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| 説明 |
Platelet-derived growth factor-B (PDGFB) is necessary for normal cardiovascular development. The administration of PDGFB alone normalized tumor vasculature by increasing periendothelial coverage and vascular functionality. Interestingly, this effect exerted by PDGFB was also observed in the presence of DAPT. So PDGFB is able to improve tumor vascularity and allows the anticancer action of DAPT in the tumor. PDGFB Protein, Human, Recombinant (His) is expressed in yeast with His tag. The predicted molecular weight is 14.3 kDa and the accession number is P01127-2. |
| Species |
Human
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| Expression Host |
Yeast
|
| Tag |
His
|
| Accession Number |
P01127-2
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| 別名 |
platelet-derived growth factor beta polypeptide, SSV, c-sis, SIS, PDGF-2, IBGC5, PDGF2, platelet-derived growth factor β polypeptide
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| Construction |
A DNA sequence encoding the human PDGFB (NP_148937.1) (Ser82-Thr190) was expressed with a polyhistidine tag at the N-terminus.
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| Protein Purity |
> 95 % as determined by SDS-PAGE.
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| 分子量 |
14.3 kDa (predicted)
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| Endotoxin |
< 1.0 EU per μg of the protein as determined by the LAL method.
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| Formulation |
Supplied as sterile PBS, 25 % Glycerol, pH 7.4. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
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| Reconstitution |
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
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| Stability & Storage |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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| Shipping |
Solution. It is shipped out with blue ice.
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| Research Background |
Platelet-derived growth factor-B (PDGFB) is necessary for normal cardiovascular development. The administration of PDGFB alone normalized tumor vasculature by increasing periendothelial coverage and vascular functionality. Interestingly, this effect exerted by PDGFB was also observed in the presence of DAPT. So PDGFB is able to improve tumor vascularity and allows the anticancer action of DAPT in the tumor.
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