PDGF R beta/CD140b Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 59 kDa and the accession number is P09619.
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
100 μg | 約5 days | ¥ 187,000 |
500 μg | 約5 days | ¥ 746,000 |
1 mg | 約5 days | ¥ 1,248,500 |
説明 | PDGF R beta/CD140b Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 59 kDa and the accession number is P09619. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | C-His-Avi |
Accession Number | P09619 |
別名 | PDGFR-2, PDGF R β/CD140b, PDGFR1, CD140B, PDGFR, PDGF R β, JTK12, PDGF R beta, PDGFR-β, PDGFRB |
Construction | Leu33-Phe530 |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 59 kDa (predicted). Due to glycosylation, the protein migrates to 78-115 kDa based on Tris-Bis PAGE result. |
Endotoxin | < 1 EU/μg by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA,PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. PDGF R plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. |
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Please read the User Guide of Recombinant Proteins for more specific information.
PDGF R beta/CD140b Protein, Human, Recombinant (His & Avi), Biotinylated PDGF R b PDGFR-2 PDGF R β/CD140b PDGFR-beta PDGFR1 JTK-12 CD140B PDGFR PDGFR-1 PDGF R β JTK 12 JTK12 PDGF R beta PDGFR 1 PDGFR-β PDGFRB PDGFR 2 PDGFR-b PDGFR2 recombinant recombinant-proteins proteins protein