PEDF Protein, Rat, Recombinant (His)

PEDF Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.9 kDa and the accession number is Q68G45.

PEDF Protein, Rat, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 124,000

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説明	PEDF Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.9 kDa and the accession number is Q68G45.

Species	Rat
Expression Host	HEK293
Tag	His
Accession Number	Q68G45
別名	serpin peptidase inhibitor, clade F，member 1
Construction	A DNA sequence encoding the rat Serpinf1 (NP_808788.1) (Met1-Thr418) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE.
分子量	45.9 kDa (predicted)

Endotoxin	< 1.0 EU per μg protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Pigment epithelium-derived factor, also known as PEDF, Serpin F1, and SERPINF1, is a multiple functional protein that has both anti-angiogenic activity and neurotrophic activity at the same time. PEDF is a secreted glycoprotein that belongs to the noninhibitory serpin. It has an alpha/beta core serine-protease inhibitor domain, three major beta-sheets, and ten alpha-helices. PEDF does not inhibit either serine or cysteine proteinases. PEDF exerts diverse physiological activities including anti-angiogenesis, anti-vasopermeability, anti-tumor, and neurotrophic activities. PEDF acts via multiple high affinity ligands and cell receptors. It has been described as a natural angiogenesis inhibitor with neurotrophic and immune-modulation properties. PEDF induces macrophages apoptosis and necrosis through the activation of peroxisome proliferator-activated receptor-gamma by which PEDF could modulate inflammatory reactions in septic shock. It balances angiogenesis in the eye and blocks tumor progression.

参考文献

1. Ren, JG. et al., 2005, Med Hypotheses. 64 (1): 74-8. 2. Filleur, S. et al., 2009. J Cell Biochem. 106 (5): 769-75. 3. Kawaguchi, T. et al., 2010, Curr Mol Med. 10 (3): 302-11. 4. Yamagishi, SI. et al., 2010, Curr Mol Med. 10 (3): 284-91. 5. Nakamura, T. et al., 2010, Curr Mol Med. 10 (3): 312-6.

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PEDF Protein, Rat, Recombinant (His)

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