Peptide Ready HLA-A*02:01&B2M Monomer Protein, Human, MHC (His & Avi), Biotinylated

HLA-A*02:01&B2M&Peptide ready Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*02:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

Peptide Ready HLA-A*02:01&B2M Monomer Protein, Human, MHC (His & Avi), Biotinylated

パッケージサイズ	在庫状況	単価(税別)
100 μg	お問い合わせ	￥ 56,000
1 mg	お問い合わせ	￥ 374,500

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説明

Species	Human
Expression Host	HEK293
Tag	C-His-Avi
Accession Number	A0A140T913(HLA-A*02:01)&P61769(B2M)
別名	HLA-A*02:01, MHC, Peptide Ready
Construction	Recombinant Biotinylated Human Peptide Ready HLA-A02:01&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305(HLA-A02:01) and Ile21-Met119(B2M).[Accession \|A0A140T913(HLA-A*02:01)&P61769(B2M)]
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Endotoxin	Less than 1EU per μg by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage	-20 to -80°C for 12 months as supplied from date of receipt.-20 to -80°C for 3-6 months in unopened state after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	HLA-A02:01&B2M&Peptide ready Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A02:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

参考文献

1. Moritz A, et al. High-throughput peptide-MHC complex generation and kinetic screenings of TCRs with peptide-receptive HLA-A*02:01 molecules. Sci Immunol. 2019 Jul 19;4(37):eaav0860. 2. Sun Y, et al. Universal open MHC-I molecules for rapid peptide loading and enhanced complex stability across HLA allotypes. bioRxiv [Preprint]. 2023 Mar 18:2023.03.18.533266.

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Peptide Ready HLA-A*02:01&B2M Monomer Protein, Human, MHC (His & Avi), Biotinylated

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