Periostin/OSF-2 Protein, Human, Recombinant (His)

Periostin/OSF-2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 86.4 kDa and the accession number is Q15063-3.

Periostin/OSF-2 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 88,500
200 μg	約5 days	￥ 151,500
500 μg	約5 days	￥ 307,500

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生物学的情報	Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. When cells are added to POSTN-His coated plates (20μg/mL, 100μL/well), approximately >45% will adhere specifically after 30 minutes at 37℃.
説明	Periostin/OSF-2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 86.4 kDa and the accession number is Q15063-3.

Species	Human
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	Q15063-3
別名	PN, OSF-2, OSF2, periostin, periostin, osteoblast specific factor, PDLPOSTN
Construction	Human POSTN isoform 3 (NP_001129407.1) corresponding to amino acid (Met 1-Gln 781)
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量	86.4 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, 5% Trehalose, pH 7.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Periostin ( POSTN ), also known as OSF2 (osteoblast specific factor 2), is a heterofunctional secreted extracellular matrix (ECM) protein comprised of four fasciclin domains that promotes cellular adhesion and movement, as well as collagen fibrillogenesis. Postn is expressed in unique growth centers during embryonic development where it facilitates epithelial-mesenchymal transition (EMT) of select cell populations undergoing reorganization. In the adult, Postn expression is specifically induced in areas of tissue injury or areas with ongoing cellular re-organization. In the adult heart Postn is induced in the ventricles following myocardial infarction, pressure overload stimulation, or generalized cardiomyopathy. Although the detailed function of Postn is still unclear, Postn-integrin interaction is thought to be involved in tumor development. Postn is frequently overexpressed in various types of human cancers, stimulating metastatic growth by promoting cancer cell survival, invasion and angiogenesis, and can be a useful marker to predict the behavior of cancer.

参考文献

1. Kudo,Y. et al., 2007, Histol Histopathol. 22 (10):1167-1174. 2. Li, J.S. et al., 2007, World J Gastroenterol. 13 (39): 5261-5266. 3. Oku, E. et al., 2008, Int J Hematol. 88 (1): 57-63. 4. HAmilton, D.W. et al., 2008, J Cell Commun Signal. 2(1-2):9-17. 5. Puglisi, F.J et al., 2008, Clin Pathol. 61 (4): 494-498. 6. Conway, S. J. et al., 2008, Curr Genomics. 9 (8): 548-555.

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Periostin/OSF-2 Protein, Human, Recombinant (His)

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