Peroxiredoxin 2 Protein, Mouse, Recombinant (His)

Peroxiredoxin 2 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 24.1 kDa and the accession number is Q61171.

Peroxiredoxin 2 Protein, Mouse, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 161,000

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説明	Peroxiredoxin 2 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 24.1 kDa and the accession number is Q61171.

Species	Mouse
Expression Host	Baculovirus-Insect Cells
Tag	His
Accession Number	Q61171
別名	Torin, TPx-B, Band-8, peroxiredoxin 2, PRP, AL022839, TR, TPx, Tdpx1, PrxII, TSA, TDX1, NkefB
Construction	A DNA sequence encoding the mouse PRDX2 (NP_035693.3) (Met1-Asn198) was expressed with a polyhistidine tag at the N-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE.
分子量	24.1 kDa (predicted)

Endotoxin	< 1.0 EU per μg protein as determined by the LAL method.
Formulation	Lyophilized from sterile 50 mM Tris, pH 7.5,150 mM NaCl,10% glycerol,0. 5 mM TCEP. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Peroxiredoxin-2, also known as Natural killer cell-enhancing factor B, NKEF-B, Thiol-specific antioxidant protein, Thioredoxin peroxidase 1, Thioredoxin-dependent peroxide reductase 1, PRDX2 and NKEFB, is a cytoplasm protein that belongs to the ahpC / TSA family. Peroxiredoxin-2 / PRDX2 contains one thioredoxin domain. Peroxiredoxin-2 / PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. Peroxiredoxin-2 / PRDX2 is not able to receive electrons from glutaredoxin. It may play an important role in eliminating peroxides generated during metabolism. Peroxiredoxin-2 / PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. The Peroxiredoxins / Prx are a family of peroxidases that can reduce H2O2 using an electron from thioredoxin (Trx) or other substances. The mammalian Peroxiredoxins / Prx family is divided into six groups (PRDX1，PRDX2, PRDX3, PRDX4, PRDX5, PRDX6) on the basis of homology of amino acid sequences. They are located in the cytosol and play a role in the cell signaling system. All six mammalian peroxiredoxins are expressed in the lung. Peroxiredoxins / Prx is overexpressed in breast cancer tissues to a great extent suggesting that Peroxiredoxins / Prx has a proliferative effect and may be related to cancer development or progression.

参考文献

1. Cha M.-K., et al., 1995, Biochem. Biophys. Res. Commun. 217:900-7. 2. Noh,D.Y. et al., 2001, Anticancer Res. 21 (3B): 2085-90. 3. Chevallet M., et al., 2003, J. Biol. Chem. 278:37146-53. 4. Schremmer,B. et al., 2007, Subcell Biochem. 44 :317-44. 5. Gauci S., et al., 2009, Anal. Chem. 81:4493-501.

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Peroxiredoxin 2 Protein, Mouse, Recombinant (His)

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