PFKM Protein, Human, Recombinant (His & GST)

PFK1, also known as PFKM, is a regulatory glycolytic enzyme. PFK1 converts fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. It is a muscle-type isozyme. There are three phosphofructokinase isozymes in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Mutations in PFK1 gene have been related to glycogen storage disease type VII, also identified as Tarui disease.

PFKM Protein, Human, Recombinant (His & GST)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 138,000

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説明

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	His, GST
Accession Number	P08237-1
別名	ATP-PFK, PFK-1, PFK1, PFKA, PFKX, GSD7, phosphofructokinase, muscle, PPP1R122
Construction	The Human PFKM (P08237-1) (Thr2-Val780) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	112.9 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 8.5, 10% glycerol.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Research Background	PFK1, also known as PFKM, is a regulatory glycolytic enzyme. PFK1 converts fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. It is a muscle-type isozyme. There are three phosphofructokinase isozymes in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Mutations in PFK1 gene have been related to glycogen storage disease type VII, also identified as Tarui disease.

参考文献

1. Yamasaki T.,et al.,(1991), Structure of the entire human muscle phosphofructokinase-encoding gene: a two-promoter system. Gene 104:277-282. 2. Sharma P.M.,et al., (1989), Cloning and expression of a human muscle phosphofructokinase cDNA.Gene 77:177-183. 3. Nakajima H.,et al.,(1987), Cloning of human muscle phosphofructokinase cDNA.FEBS Lett. 223:113-116.

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PFKM Protein, Human, Recombinant (His & GST)

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