PLA2G1B Protein, Mouse, Recombinant (His)

PLA2G1B Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 16.3 kDa and the accession number is Q9Z0Y2.

PLA2G1B Protein, Mouse, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
20 μg	約5 days	￥ 91,500

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説明	PLA2G1B Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 16.3 kDa and the accession number is Q9Z0Y2.

Species	Mouse
Expression Host	HEK293 Cells
Tag	His
Accession Number	Q9Z0Y2
別名	Pla2a, sPLA2IB, phospholipase A2, group IB (pancreas)
Construction	The Mouse PLA2G1B (NP_035237.1) extracellular domain (Met 1-Cys 146) was fused with a polyhistidine tag at the C-terminus.
Protein Purity	> 96 % as determined by SDS-PAGE
分子量	16.3 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	phospholipase A2, also known as Phosphatidylcholine 2-acylhydrolase 1B, Group IB phospholipase A2, PLA2 and PLA2G1B, is a secreted protein that belongs to the phospholipase A2 family. Phospholipase A2 / PLA2G1B catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic Phospholipase A2 / PLA2G1B enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of Phospholipase A2 / PLA2G1B have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets. Mice lacking in Phospholipase A2 / PLA2G1B are resistant to obesity and diabetes induced by feeding a diabetogenic high-fat/high-carbohydrate diet. Oral supplementation of a diabetogenic diet with the PLA2G1B inhibitor methyl indoxam effectively suppresses diet-induced obesity and diabetes. PLA2G1B inhibition may be a potentially effective oral therapeutic option for treatment of obesity and diabetes.

参考文献

1. Labonté,E.D. et al., 2006, Diabetes. 55 (4) :935-41. 2. Mounier,C.M. et al.,2008, Br J Cancer. 98 (3):587-95. 3. Hui,D.Y. et al., 2009, Br J Pharmacol. 157 (7):1263-9. 4. Labonté,E.D. et al., 2010, FASEB J. 24 (7):2516-24.

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PLA2G1B Protein, Mouse, Recombinant (His)

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