PLBL2/PLBD2 Protein, Chinese hamster, Recombinant (His)

PLBL2/PLBD2 Protein, Chinese hamster, Recombinant (His) is expressed in CHO mammalian cells with His tag. The predicted molecular weight is 63.3 kDa and the accession number is G3I6T1-1.

PLBL2/PLBD2 Protein, Chinese hamster, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 184,000
1 mg	約5 days	￥ 1,198,000

バルクのお問い合わせ

説明	PLBL2/PLBD2 Protein, Chinese hamster, Recombinant (His) is expressed in CHO mammalian cells with His tag. The predicted molecular weight is 63.3 kDa and the accession number is G3I6T1-1.

Species	Chinese hamster
Expression Host	CHO Cells
Tag	C-His
Accession Number	G3I6T1-1
別名	phospholipase B domain containing 2
Construction	The chinese hamster PLBD2 (G3I6T1-1) (Leu38-Asp585) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量	63.3 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	PLBD2 localizes to the lysosome, as its absence could plausibly lead to a serious yet unrecognized lysosomal storage disease. PLBD1 and PLBD2 are semi-orphans in the sense of being probable phospholipases of B class but with uncertain physiological substrates and thus functionalities. PLBD1 and PLBD2 constitute a small gene family (sequence homology class) within vertebrates though one that occurs expanded in some early diverging eukaryotes. PLBD2 presents a special difficulty in that a sequence of post-translational steps are apparently necessary for its activation. Without these, potential substrates can hardly be assayed. These steps include removal of the signal peptide, mannosylation appropriate to the lysosome targeting receptor, and self-catalytic proteolytic activation to expose the substrate binding site as this becomes appropriate.

参考文献

1. Morgan CP. et al., 2004), Biochem J. 382 (2): 441-9. 2. Kim W. et al., 2011, Mol Cell. 44 (2): 325-40. 3. Havugimana PC. et al., 2012. Cell. 150 (5): 1068-81.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

PLBL2/PLBD2 Protein, Chinese hamster, Recombinant (His)

参考文献

Calculator

技術サポート

Keywords