SARS-CoV-2 PLpro/papain-like protease Protein (His & Avi)

The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. Although the primary function of PLpro and 3CLpro are to process the viral polyprotein in a coordinated manner, PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses. SARS-CoV-2 PLpro/papain-like protease Protein (His & Avi) is expressed in E. coli expression system with N-His-Avi tag. The predicted molecular weight is 38.6 kDa and the accession number is P0C6U8.

SARS-CoV-2 PLpro/papain-like protease Protein (His & Avi)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 53,000
500 μg	約5 days	￥ 214,000
1 mg	約5 days	￥ 356,000

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説明

Species	SARS
Expression Host	E. coli
Tag	N-His-Avi
Accession Number	P0C6U8
別名	Plpro, PL2-PRO, pp1a, nsp1, Replicase polyprotein 1a, Papain-like Protease, PL-PRO
Construction	Recombinant SARS-CoV-2 PLpro/papain-like protease Protein is expressed from E.coil with His tag and Avi tag at the N-Terminus. It contains Glu1-Ile314.[Accession \|P0C6U8]
Protein Purity	> 95% as determined by Tris-Bis PAGE
分子量	The protein has a predicted MW of 38.6 kDa same as Tris-Bis PAGE result.

Endotoxin	Less than 1EU per μg by the LAL method.
Formulation	Supplied as 0.22μm filtered solution in 20mM Tris, 100mM NaCl, 10% Glycerol (pH 7.8).
Stability & Storage	Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. Although the primary function of PLpro and 3CLpro are to process the viral polyprotein in a coordinated manner, PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses.

参考文献

1. Báez-Santos YM, et al. The SARS-coronavirus papain-like protease: structure, function and inhibition by designed antiviral compounds. Antiviral Res. 2015 Mar;115:21-38. doi: 10.1016/j.antiviral.2014.12.015. Epub 2014 Dec 29. PMID: 25554382; PMCID: PMC5896749.

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SARS-CoV-2 PLpro/papain-like protease Protein (His & Avi)

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