PMVK Protein, Human, Recombinant (His)

PMVK is a peroxisomal enzyme that catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate, the fifth reaction of the cholesterol biosynthetic pathway. Studies in rat show that the message level and the enzyme activity of PMVK is regulated by sterol, and that this regulation is coordinated with 3-hydroxy-3-methylglutaryl coenzyme A reductase, the rate-limiting enzyme of cholesterol biosynthesis.

PMVK Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 91,500

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説明

Species	Human
Expression Host	E. coli
Tag	N-His
Accession Number	Q15126
別名	PMK, PMKA, phosphomevalonate kinase, HUMPMKI, PMKASE
Construction	mature form of Human PMVK(Q15126) (Met1-Leu192)
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	23.8 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Supplied as sterile 50 mM MOPS, 150 mM KCl, 1 mM DTT, pH 7.0.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Research Background	PMVK is a peroxisomal enzyme that catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate, the fifth reaction of the cholesterol biosynthetic pathway. Studies in rat show that the message level and the enzyme activity of PMVK is regulated by sterol, and that this regulation is coordinated with 3-hydroxy-3-methylglutaryl coenzyme A reductase, the rate-limiting enzyme of cholesterol biosynthesis.

参考文献

1. Chambliss K.L., et al.,(1996), Molecular cloning of human phosphomevalonate kinase and identification of a consensus peroxisomal targeting sequence. J. Biol. Chem. 271:17330-17334. 2. Olivier L.M., et al., (1999), Characterization of phosphomevalonate kinase: chromosomal localization, regulation, and subcellular targeting.J. Lipid Res. 40:672-679. 3. Herdendorf T.J., et al.,(2006), Phosphomevalonate kinase: functional investigation of the recombinant human enzyme. Biochemistry 45:3235-3242.

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PMVK Protein, Human, Recombinant (His)

参考文献

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