PPM1A Protein, Human, Recombinant (His)

PPM1A Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 43.8 kDa and the accession number is P35813-1.

PPM1A Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 138,000

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説明	PPM1A Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 43.8 kDa and the accession number is P35813-1.

Species	Human
Expression Host	E. coli
Tag	His
Accession Number	P35813-1
別名	protein phosphatase, Mg2+/Mn2+ dependent, 1A, PP2Cα, PP2C-ALPHA, PP2CA, PP2Calpha, PP2C-α
Construction	The Human PPM1A isoform alpha-1 (P35813-1) (Met 1-Trp 382) was fused with a polyhistidine tag at the C-terminus.
Protein Purity	> 85 % as determined by SDS-PAGE
分子量	43.8 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, 10% glycerol, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Protein phosphatase 1A (PPM1A / PP2CA) is an enzyme belonging to the PP2C family of Ser / Thr protein phosphatases. Members of PP2C family are negative regulators of cell stress response pathways and the MAP kinases and MAP kinase kinases. It has also been demonstrated to inhibit the activation of p38 and JNK kinase cascades. PPM1A dephosphorylates and promotes nuclear export of TGFβ-activated Smad2/3. Ectopic expression of PPM1A abolishes TGFβ-induced antiproliferative and transcriptional responses, whereas depletion of PPM1A enhances TGFβ signaling in mammalian cells. It has been demonstrated that PPM1A / PP2CA, through dephosphorylation of Smad2/3, plays a critical role in terminating TGFβ signaling. Overexpression of PPM1A is reported to activate the expression of the tumor suppressor gene TP53 / p53, which leads to cell apoptosis.

参考文献

1. Lin X,et al.(2006) PPM1A functions as a Smad phosphatase to terminate TGFbeta signaling. Cell. 125(5): 915-28. 2. Marc F,et al.(2003) Protein phosphatase 2C binds selectively to and dephosphorylates metabotropic glutamate receptor 3. Proc Natl Acad. 100 (26): 16006-11. 3. Mann DJ,et al.(1992) Mammalian protein serine/threonine phosphatase 2C: cDNA cloning and comparative analysis of amino acid sequences. Biochim Biophys Acta. 1130 (1): 100-4.

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PPM1A Protein, Human, Recombinant (His)

参考文献

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