PPM1A Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 43.3 kDa and the accession number is P49443.
説明 | PPM1A Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 43.3 kDa and the accession number is P49443. |
Species | Mouse |
Expression Host | E. coli |
Tag | His |
Accession Number | P49443 |
別名 | AU017636, AI427932, MMPa-2, MPPa-1, protein phosphatase, Mg2+/Mn2+ dependent, 1A, 2310003C21Rik, 2900017D14Rik |
Construction | The Mouse PPM1A (NP_032936.1) (Met 1-Trp 382) was expressed, with a polyhistide tag at the C-terminus. |
Protein Purity | > 98 % as determined by SDS-PAGE |
分子量 | 43.3 kDa (predicted) |
Endotoxin | Please contact us for more information. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 10 mM HEPES, 500 NaCl, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Protein phosphatase 1A (PPM1A / PP2CA) is an enzyme belonging to the PP2C family of Ser / Thr protein phosphatases. Members of PP2C family are negative regulators of cell stress response pathways and the MAP kinases and MAP kinase kinases. It has also been demonstrated to inhibit the activation of p38 and JNK kinase cascades. PPM1A dephosphorylates and promotes nuclear export of TGFβ-activated Smad2/3. Ectopic expression of PPM1A abolishes TGFβ-induced antiproliferative and transcriptional responses, whereas depletion of PPM1A enhances TGFβ signaling in mammalian cells. It has been demonstrated that PPM1A / PP2CA, through dephosphorylation of Smad2/3, plays a critical role in terminating TGFβ signaling. Overexpression of PPM1A is reported to activate the expression of the tumor suppressor gene TP53 / p53, which leads to cell apoptosis. |
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PPM1A Protein, Mouse, Recombinant (His) AU-017636 AU017636 AI427932 AI 427932 AU 017636 AI-427932 MMPa-2 MPPa-1 protein phosphatase, Mg2+/Mn2+ dependent, 1A 2310003C21Rik 2900017D14Rik recombinant recombinant-proteins proteins protein