PRKAR1A Protein, Mouse, Recombinant (His)

PRKAR1A Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 44.6 kDa and the accession number is Q9DBC7.

PRKAR1A Protein, Mouse, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 161,000

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説明	PRKAR1A Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 44.6 kDa and the accession number is Q9DBC7.

Species	Mouse
Expression Host	Baculovirus Insect Cells
Tag	C-His
Accession Number	Q9DBC7
別名	1300018C22Rik, protein kinase, cAMP-dependent, regulatory, type I, alpha, RIalpha, Tse1, RIα, Tse-1, protein kinase, cAMP-dependent, regulatory, type I, α
Construction	The Mouse PRKAR1A (NP_068680.1) (Met1-Val381) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE.
分子量	44.6 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 300 mM NaCl, pH 7.5, 10% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	PRKAR1A, also known as PRKAR1 and PKR1, is one of the regulatory subunits of cAMP-dependent protein kinase A (PKA). PKA can be activated by cAMP. cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating PKA, which transduces the signal throughphosphorylation of different target proteins. The inactive holoenzyme of PKA is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of PKA have been identified in humans. PRKAR1A was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids Three alternatively spliced transcript variants encoding the same protein have been observed.

参考文献

1. Huang L J. et al., 1997, Proc Natl Acad Sci. 94 (21): 11184-9. 2. Herberg F W. et al., 2000, J Mol Biol. 298 (2): 329-39. 3. Scambler P. et al., 1987, Am J Hum Genet. 41 (5): 925-32.

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PRKAR1A Protein, Mouse, Recombinant (His)

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