Prostasin/PRSS8 Protein, Mouse, Recombinant (aa 30-289, His)

Prostasin/PRSS8 Protein, Mouse, Recombinant (aa 30-289, His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 29.3 kDa and the accession number is EDL17608.1.

Prostasin/PRSS8 Protein, Mouse, Recombinant (aa 30-289, His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 138,000

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説明	Prostasin/PRSS8 Protein, Mouse, Recombinant (aa 30-289, His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 29.3 kDa and the accession number is EDL17608.1.

Species	Mouse
Expression Host	Baculovirus Insect Cells
Tag	His
Accession Number	EDL17608.1
別名	C79772, CAP1, protease, serine, 8, fr, AI313909, 2410039E18Rik, mCAP1
Construction	The Mouse PRSS8 (EDL17608.1 ) (Ala 30-Gln 289) was expressed，fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
Protein Purity	> 97 % as determined by SDS-PAGE
分子量	29.3 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Prostasin (Prss8), also known as channel activating protease 1 (CAP1), is a trypsinlike serine peptidase, and plays important roles in epithelial physiology. It is originally purified as an active, soluble enzyme from human seminal fluid and is highly expressed in prostate, lung, kidney, salivary gland and pancreas. Prostasin is expressed as a glycosyl-phosphatidylinositol (GPI)-anchored membrane protein in prostate epithelial cells, and also exists as a secreted proteolytic enzyme possibly via tryptic cleavage of its COOH-terminal hydrophobic domain. Prostasin is found to activate the epithelial sodium channel (ENaC) which is tightly regulated and is critical for maintaining salt and fluid balance in the lung and kidney in both normal and pathological conditions. Accordingly, prostasin has been proposed as a target for therapeutic inhibition in cystic fibrosis. Besides, prostasin inhibits prostate and breast cancer cell invasion in vitro, suggesting a functional role as a suppressor of tumor invasion, as well as a regulator of gene expression during inflammation.

参考文献

1. Yu J.X.,et al.,(1995), Molecular cloning, tissue-specific expression, and cellular localization of human prostasin mRNA. J. Biol. Chem. 270:13483-13489. 2. Ota T.,et al., (2004), Complete sequencing and characterization of 21,243 full-length human cDNAs.Nat. Genet. 36:40-45. 3. Martin J.,et al.,(2004), The sequence and analysis of duplication-rich human chromosome 16.Nature 432:988-994.

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Prostasin/PRSS8 Protein, Mouse, Recombinant (aa 30-289, His)

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