PSGL-1/CD162 Protein, Human, Recombinant (His)

PSGL-1/CD162 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 30.5 kDa and the accession number is Q14242-1.

PSGL-1/CD162 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 88,500
200 μg	約5 days	￥ 156,500
500 μg	約5 days	￥ 330,500

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生物学的情報	Testing in progress
説明	PSGL-1/CD162 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 30.5 kDa and the accession number is Q14242-1.

Species	Human
Expression Host	HEK293 Cells
Tag	His
Accession Number	Q14242-1
別名	selectin P ligand, PSGL-1, PSGL1, CD162, CLA
Construction	Human SELPLG (Q14242-1) (Met1-Gly295) with a C-terminal polyhistidine tag
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
分子量	30.5 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinity ounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~12 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin.

参考文献

1. Sako, D., et al. 1993, Cell. 75: 1179-1186.2. 2. Wilkins, P. P. et al., 1995, J. Biol. Chem. 270: 22677-22680.3. 3. Frenette, P. S. et al., 2000, J. Exp. Med. 191: 1413-1422.4. 4. Vandendries, E.R .et al., 2004, Thromb. Haemost. 92: 459-466..5. 5. Pouyani, T. et al., 1995, Cell. 83: 333-343.

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PSGL-1/CD162 Protein, Human, Recombinant (His)

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