PSGL-1/CD162 Protein, Human, Recombinant (mFc)

PSGL-1/CD162 Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 55.4 kDa and the accession number is AAC50061.1.

PSGL-1/CD162 Protein, Human, Recombinant (mFc)

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 52,500
100 μg	約5 days	￥ 93,000
200 μg	約5 days	￥ 164,500
500 μg	約5 days	￥ 349,000

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生物学的情報	Testing in progress
説明	PSGL-1/CD162 Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 55.4 kDa and the accession number is AAC50061.1.

Species	Human
Expression Host	HEK293
Tag	mFc
Accession Number	AAC50061.1
別名	PSGL-1, CLA, selectin P ligand, PSGL1, CD162
Construction	A DNA sequence encoding the human SELPLG (AAC50061.1) (Met1-Val295) was expressed with the Fc region of mouse IgG1 at the C-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE.
分子量	55.4 kDa (predicted)

Endotoxin	< 1.0 EU per μg protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinity ounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~12 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin.

参考文献

1. Sako, D., et al. 1993, Cell. 75: 1179-1186.2. 2. Wilkins, P. P. et al., 1995, J. Biol. Chem. 270: 22677-22680.3. 3. Frenette, P. S. et al., 2000, J. Exp. Med. 191: 1413-1422.4. 4. Vandendries, E.R .et al., 2004, Thromb. Haemost. 92: 459-466..5. 5. Pouyani, T. et al., 1995, Cell. 83: 333-343.

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PSGL-1/CD162 Protein, Human, Recombinant (mFc)

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