PSGL-1/CD162 Protein, Mouse, Recombinant (hFc)

PSGL-1/CD162 Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 57.4 kDa and the accession number is Q62170.

PSGL-1/CD162 Protein, Mouse, Recombinant (hFc)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 138,000
1 mg	約5 days	￥ 897,500

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説明	PSGL-1/CD162 Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 57.4 kDa and the accession number is Q62170.

Species	Mouse
Expression Host	HEK293 Cells
Tag	hFc
Accession Number	Q62170
別名	Psgl1, Psgl-1, CD162, selectin P ligand, Selp1, Selpl
Construction	The extracellular domain of Mouse SELPLG (Q62170) (Met 1-Cys 307) was fused with the Fc region of Human IgG1 at the C-terminus.
Protein Purity	> 85 % as determined by SDS-PAGE
分子量	57.4 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinity ounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~12 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin.

参考文献

1. Sako, D., et al. 1993, Cell. 75: 1179-1186.2. 2. Wilkins, P. P. et al., 1995, J. Biol. Chem. 270: 22677-22680.3. 3. Frenette, P. S. et al., 2000, J. Exp. Med. 191: 1413-1422.4. 4. Vandendries, E.R .et al., 2004, Thromb. Haemost. 92: 459-466..5. 5. Pouyani, T. et al., 1995, Cell. 83: 333-343.

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PSGL-1/CD162 Protein, Mouse, Recombinant (hFc)

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