PSMP Protein, Human, Recombinant (hFc)

PSMP Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 37.9 kDa and the accession number is Q1L6U9.

PSMP Protein, Human, Recombinant (hFc)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 96,000
500 μg	約5 days	￥ 383,500
1 mg	約5 days	￥ 643,000

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説明	PSMP Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 37.9 kDa and the accession number is Q1L6U9.

Species	Human
Expression Host	HEK293 Cells
Tag	C-hFc
Accession Number	Q1L6U9
別名	PC3-secreted microprotein, Msmp, Psmp
Construction	Lys37-Ser139
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	37.9 kDa (predicted). Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	PC3-secreted microprotein (PSMP)/microseminoprotein (MSMP) is a novel chemotactic cytokine and its receptor is CCR2. PSMP was highly expressed in fibrotic/cirrhotic tissues from patients with different etiologies of liver disease and in the 3 experimental mouse models of fibrosis. Damage-associated molecular pattern molecules HMGB-1 and IL-33 induced hepatocytes to produce PSMP. PSMP promotes liver fibrosis through inflammatory macrophage infiltration, polarization and production of proinflammatory cytokines, as well as direct activation of hepatic stellate cells via its receptor CCR2.

参考文献

1. She S, et al. PSMP/MSMP promotes hepatic fibrosis through CCR2 and represents a novel therapeutic target. J Hepatol. 2020 Mar;72(3):506-518. doi: 10.1016/j.jhep.2019.09.033. Epub 2019 Dec 6. PMID: 31813573.

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PSMP Protein, Human, Recombinant (hFc)

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