PTK9 Protein, Human, Recombinant

PTK9 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 29 kDa and the accession number is Q12792-4.

PTK9 Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 161,000

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説明	PTK9 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 29 kDa and the accession number is Q12792-4.

Species	Human
Expression Host	E. coli
Tag	Tag Free
Accession Number	Q12792-4
別名	MGC41876, twinfilin actin binding protein 1, A6, MGC23788, PTK9
Construction	The Human TWF1 isoform 4 (Q12792-4) (Met 1-Asp 252) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.
Protein Purity	> 94 % as determined by SDS-PAGE
分子量	29 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4, 10% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Twinfilin-1, also known as Protein A6, Protein tyrosine kinase 9, TWF1 and PTK9, is a cytoplasm protein that belongs to the actin-binding proteins ADF family and Twinfilin subfamily. Twinfilin-1 (TWF1 / PTK9 ) is a highly conserved actin monomer-binding protein that regulates cytoskeletal dynamics in organisms from yeast to mammals. In addition to the mammalian twinfilin-1, a second protein with approximately 65% sequence identity to twinfilin-1 exists in mouse and humans. TWF1 / PTK9 is expressed at high levels in the colon, testis, ovary, prostate and lung. It is expressed at lower levels in the brain, bladder and heart. It is not detected in liver. TWF1 / PTK9 is an actin-binding protein involved in motile and morphological processes. It inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. TWF1 / PTK9 seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.

参考文献

1. Beeler JF, et al.,1994, Mol Cell Biol 14 (2): 982-8. 2. Palmgren S, et al., 2002, J. Cell. Sci. 115 (Pt 5): 881-6. 3. Vartiainen MK, et al.,2003, J. Biol. Chem. 278 (36): 34347-55. 4. Hassel S, et al.,2004, Proteomics 4 (5): 1346-58. 5. Moseley,J.B. et al., 2006, J Cell Sci. 119 (Pt 8):1547-57.

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PTK9 Protein, Human, Recombinant

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