PVRL1/NECTIN1 Protein, Human, Recombinant (hFc), Biotinylated

PVRL1/NECTIN1 Protein, Human, Recombinant (hFc), Biotinylated is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 61 kDa and the accession number is Q15223-1.

PVRL1/NECTIN1 Protein, Human, Recombinant (hFc), Biotinylated

パッケージサイズ	在庫状況	単価(税別)
20 μg	在庫あり	￥ 45,500
100 μg	約5 days	￥ 107,500
200 μg	約5 days	￥ 183,000
500 μg	約5 days	￥ 369,500

バルクのお問い合わせ

生物学的情報	Testing in progress
説明	PVRL1/NECTIN1 Protein, Human, Recombinant (hFc), Biotinylated is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 61 kDa and the accession number is Q15223-1.

Species	Human
Expression Host	HEK293 Cells
Tag	hFc
Accession Number	Q15223-1
別名	PRR1, ED4, PVRR, nectin-1, OFC7, SK-12, CD111, CLPED1, HVEC, poliovirus receptor-related 1 (herpesvirus entry mediator C), PRR, HV1S, PVRR1, HIgR
Construction	The Human PVRL1 isoform 1 (NP_002846.3) extracellular domain (Met 1-Thr 334) was was fused with the Fc region of Human IgG1 at the C-terminus. The purified protein was biotinylated in vitro.
Protein Purity	> 95 % as determined by SDS-PAGE.
分子量	61 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Poliovirus receptor-related 1 (herpesvirus entry mediator C; nectin-1; CD111), also known as PVRL1 is a cell adhesion molecule belonging to the immunoglobulin superfamily that can bind to virion glycoprotein D (gD) to mediate entry of herpes simplex viruses (HSV) and pseudorabies virus (PRV). CD111/Nectin-1/PVRL1 colocalizes with E-cadherin at adherens junctions in epithelial cells. The disruption of cell junctions can result in the redistribution of nectin-1. To determine whether disruption of junctions by calcium depletion influenced the susceptibility of epithelial cells to viral entry, Madin-Darby canine kidney cells expressing endogenous nectin-1 or transfected human nectin-1 were tested for the ability to bind soluble forms of viral gD and to be infected by HSV and PRV, before and after calcium depletion. It has been revealed that binding of HSV and PRV gD was localized to adherens junctions in cells maintained in normal medium but was distributed, along with nectin-1, over the entire cell surface after calcium depletion. Both the binding of gD and the fraction of cells that could be infected by HSV-1 and PRV were enhanced by calcium depletion. Taken together, CD111/Nectin-1/PVRL1 confined to adherens junctions in epithelial cells is not very accessible to virus, whereas dissociation of cell junctions releases nectin-1 to serve more efficiently as an entry recptor.

参考文献

1. Yoon M,et al.(2002) Disruption of adherens junctions liberates nectin-1 to serve as receptor for herpes simplex virus and pseudorabies virus entry. J Virol. 76(14): 7203-8. 2. Mata M,et al.(2001) HveC (nectin-1) is expressed at high levels in sensory neurons, but not in motor neurons, of the rat peripheral nervous system. J Neurovirol. 7(5): 476-80. 3. Haarr L,et al.(2001) Transcription from the gene encoding the herpesvirus entry receptor nectin-1 (HveC) in nervous tissue of adult mouse. Virology. 287(2): 301-9.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

PVRL1/NECTIN1 Protein, Human, Recombinant (hFc), Biotinylated

参考文献

Calculator

技術サポート

Keywords