RAB27B Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 50.8 kDa and the accession number is O00194.
| 説明 |
RAB27B Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 50.8 kDa and the accession number is O00194. |
| Species |
Human
|
| Expression Host |
HEK293 Cells
|
| Tag |
N-mFc
|
| Accession Number |
O00194
|
| 別名 |
RAB27B, RAB27B, member RAS oncogene family, C25KG
|
| Construction |
The Human RAB27B (O00194) (Thr2-Lys215) was expressed with the Fc region of Mouse IgG1 at the N-terminus.
|
| Protein Purity |
> 95 % as determined by SDS-PAGE
|
| 分子量 |
50.8 kDa (predicted)
|
| Endotoxin |
< 1.0 EU/μg of the protein as determined by the LAL method.
|
| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
|
| Reconstitution |
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
|
| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
|
| Shipping |
In general, Lyophilized powders are shipping with blue ice.
|
| Research Background |
RAB27B is a member of the Rab family GTPases involved in vesicle trafficking. The Rab27 subfamily consists of Rab27a and Rab27b. The human RAB27B gene is organized in six exons, spanning about 69 kb in the chromosome 18q21.1 region. The Rab27 subfamily of Ras-like GTPases is highly conserved in mammals. There is high degree of conservation in sequence and gene structure between RAB27A and RAB27B genes. Exogenous expression of Rab27b in melanocytes results in melanosomal association as observed for Rab27a, suggesting the two Rab27 proteins are functional homologues. As with RAB27A in Griscelli Disease, RAB27B may be also associated with human disease mapping to chromosome 18.
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