RANKL/TNFSF11/CD254 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with N-6xHis tag. The predicted molecular weight is 30-35 KDa and the accession number is BAA97257.1.
パッケージサイズ | 在庫状況 | 単価(税別) |
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10 μg | 在庫あり | ¥ 27,000 |
50 μg | 約5 days | ¥ 80,500 |
500 μg | 約5 days | ¥ 280,000 |
1 mg | 約5 days | ¥ 682,000 |
生物学的情報 |
Loaded Recombinant Human OPG-Fc on Pro A Biosensor, can bind Mouse RANKL-His with an affinity constant of 1.02 pM as determined in BLI assay. (Regularly tested)
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説明 | RANKL/TNFSF11/CD254 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with N-6xHis tag. The predicted molecular weight is 30-35 KDa and the accession number is BAA97257.1. |
Species | Mouse |
Expression Host | Human Cells |
Tag | N-6His |
Accession Number | BAA97257.1 |
別名 | Receptor activator of nuclear factor κ-B ligand, Osteoprotegerin ligand, OPGL, TNF-related activation-induced cytokine, CD254, Receptor activator of nuclear factor kappa-B ligand, ODF, RANKL, TRANCE, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Tnfsf11 |
Amino Acid | Arg43-Asp287 |
Construction | Recombinant Mouse TNF-related Activation-induced Cytokine is produced by our Mammalian expression system and the target gene encoding Arg43-Asp287 is expressed with a 6His tag at the N-terminus. |
Protein Purity |
Greater than 90% as determined by reducing SDS-PAGE. Greater than 90% as determined by SEC-HPLC.
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分子量 | 30-35 KDa, reducing conditions |
Endotoxin | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM Hepes-NaOH, 50mM NaCl, 6% Trehalose, 4% Mannitol, 0.05% Tween 80, pH 8.0. |
Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Stability & Storage |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
Shipping |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
Research Background | Mouse tumor necrosis factor ligand superfamily member 11(Tnfsf11) is a member of the tumor necrosis factor (TNF) cytokine family. Tnfsf11 is widely expressed in cells including T cells and T cell rich organs, such as thymus and lymph nodes. This cytokine can bind to TNFRSF11B/OPG andTNFRSF11A/RANK. Tnfsf11 is involved in a number of fundamental biological processes such as acting as regulator of interactions between T-cells and dendritic cells, the regulation of the T-cell-dependent immune response and enhancing bone-resorption in humoral hypercalcemia of malignancy. It augments the ability of dendritic cells to stimulate naive T-cell proliferation. |
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Please read the User Guide of Recombinant Proteins for more specific information.
RANKL/TNFSF11/CD254 Protein, Mouse, Recombinant (His) Receptor activator of nuclear factor k-B ligand CD-254 Receptor activator of nuclear factor κ-B ligand Osteoprotegerin ligand OPGL TNF-related activation-induced cytokine CD254 Receptor activator of nuclear factor kappa-B ligand ODF RANKL TRANCE Tumor necrosis factor ligand superfamily member 11 CD 254 Osteoclast differentiation factor Tnfsf11 recombinant recombinant-proteins proteins protein