RBP4 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.8 kDa and the accession number is Q00724.
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
100 μg | 在庫あり | ¥ 88,500 |
200 μg | 約5 days | ¥ 151,500 |
500 μg | 約5 days | ¥ 307,500 |
生物学的情報 | Measured by its ability to bind alltrans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4. The 50% binding concentration (BC50) is >0.5 µM. |
説明 | RBP4 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.8 kDa and the accession number is Q00724. |
Species | Mouse |
Expression Host | HEK293 Cells |
Tag | His |
Accession Number | Q00724 |
別名 | Rbp-4, retinol binding protein 4, plasma |
Construction | The Mouse RBP4 (NP_035385.1) (Met 1-Leu 201) was fused with a polyhistidine tag at the C-terminus. |
Protein Purity |
> 95 % as determined by SDS-PAGE
|
分子量 | 22.8 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a β-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM). |
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RBP4 Protein, Mouse, Recombinant (His) Rbp-4 retinol binding protein 4, plasma recombinant recombinant-proteins proteins protein