IGF1/IGF-I Protein, Human, Recombinant

IGF1/IGF-I Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 7.8 KDa. and the accession number is P05019-1.

IGF1/IGF-I Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 13,000

バルクのお問い合わせ

生物学的情報

1.Measured by its binding ability in a functional ELISA. Immobilized Human IGF1 at 2 μg/ml (100 μl/well) can bind Human IGFBP4 His, the EC50 of Human IGFBP4 His is 10-60 ng/mL. 2. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 3.5-14 ng/mL. 3.Human breast cancer organoids were cultured with FGF7, RSPO1, IGF1, EGF, NRG1 Beta 1, NOG. (Routinely tested). 4.Human breast organoids were cultured with IGF1, NRG1 Beta 1. (Routinely tested). 5.Human ovarian organoids were cultured with IGF1, NRG1 Beta 1, RSPO1, EGF, NOG. (Routinely tested).

説明

IGF1/IGF-I Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 7.8 KDa. and the accession number is P05019-1.

Species	Human
Expression Host	E. coli
Tag	Tag Free
Accession Number	P05019-1
別名	insulin-like growth factor 1, somatomedin C, MGF, IGF-I, IGF-1, IGF‑I, IGFI, IGF1A
Construction	Human IGF1 (P05019-1) (Gly49-Ala118)
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	The recombinant human IGF1 consists of 71 amino acids and has a calculated molecular mass of 7.8 KDa.

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 30% Acetonitrile, 0.1% TFA . Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	IGF I, also known as Mechano Growth Factor, somatomedin-C, IGF-I, and IGF1, is a secreted protein that belongs to the insulin family. The insulin family, comprised of insulin, relaxin, insulin-like growth factors I and II ( IGF-I and IGF-II ), and possibly the beta-subunit of 7S nerve growth factor, represents a group of structurally related polypeptides whose biological functions have diverged. The IGFs, or somatomedins, constitute a class of polypeptides that have a key role in pre-adolescent mammalian growth. IGF-I expression is regulated by GH and mediates postnatal growth, while IGF-II appears to be induced by placental lactogen during prenatal development. IGF1 / IGF-I may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. IGF1 / IGF-I stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also about enhancing glucose uptake. Defects in IGF1 / IGF-I are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness, and mental retardation.

参考文献

1. Jansen M., et al.,(1983), Sequence of cDNA encoding human insulin-like growth factor I precursor. Nature 306:609-611. 2. de Pagter-Holthuizen P., et al., (1986), Organization of the human genes for insulin-like growth factors I and II.FEBS Lett. 195:179-184. 3. le Bouc Y., et al.,(1986), Complete characterization of the human IGF-I nucleotide sequence isolated from a newly constructed adult liver cDNA library.FEBS Lett. 196:108-112.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

IGF1/IGF-I Protein, Human, Recombinant

参考文献

Calculator

技術サポート

Keywords