R-Spondin 1/RSPO1 Protein, Human, Recombinant (aa 1-146, His)

R-Spondin 1/RSPO1 Protein, Human, Recombinant (aa 1-146, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 14 kDa and the accession number is Q2MKA7-1.

R-Spondin 1/RSPO1 Protein, Human, Recombinant (aa 1-146, His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 142,000
1 mg	約5 days	￥ 922,500

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説明	R-Spondin 1/RSPO1 Protein, Human, Recombinant (aa 1-146, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 14 kDa and the accession number is Q2MKA7-1.

Species	Human
Expression Host	HEK293 Cells
Tag	His
Accession Number	Q2MKA7-1
別名	R-Spondin 1, CRISTIN3, RSPO
Construction	The amino acids (Met 1-Ala 146) of Human RSPO1 (NP_001033722.1) was expressed, fused with a polyhistidine tag at the C-terminus.
Protein Purity	> 98 % as determined by SDS-PAGE
分子量	14 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.

参考文献

1. Kamata T, et al. (2004) R-spondin, a novel gene with thrombospondin type 1 domain, was expressed in the dorsal neural tube and affected in Wnts mutants. Biochim Biophys Acta. 1676(1):51-62. 2. Ota T, et al. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 36(1):40-5. 3. Strausberg RL, et al. (2003) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):16899-903.

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R-Spondin 1/RSPO1 Protein, Human, Recombinant (aa 1-146, His)

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