R-Spondin 1/RSPO1 Protein, Human, Recombinant

R-Spondin 1/RSPO1 Protein, Human, Recombinant is expressed in CHO mammalian cells. The predicted molecular weight is 26.8 kDa and the accession number is Q2MKA7-1.

R-Spondin 1/RSPO1 Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
20 μg	在庫あり	￥ 41,000
100 μg	約5 days	￥ 114,000
200 μg	約5 days	￥ 223,500
500 μg	約5 days	￥ 633,500

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生物学的情報

1. Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 10-80 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.
2. Human intestinal cancer organoids were cultured with RSPO1, EGF, NOG. (Routinely tested).
3. Human colorectum organoids were cultured with RSPO1, EGF, NOG. (Routinely tested).
4. Human lung cancer organoids were cultured with FGF2, FGF4, FGF7, EGF, FGF10, NOG, RSPO1. (Routinely tested).
5. Human lung organoids were cultured with FGF2, FGF4, FGF7,EGF, FGF10, NOG, RSPO1. (Routinely tested).
6. Human cholangiocarcinomas organoids were cultured with FGF2, HGF, FGF7, EGF, FGF10, NOG, RSPO1. (Routinely tested).
7. Human liver cancer organoids were cultured with FGF2, HGF, FGF7, EGF, FGF10, TGFB1, NOG,RSPO1. (Routinely tested).
8. Human kidney cancer organoids were cultured with FGF2, FGF7, EGF, FGF10, NOG, RSPO1. (Routinely tested).
9. Human kidney organoids were cultured with FGF7, EGF, FGF10,NOG, RSPO1, HGF, FGF4. (Routinely tested).
10. Human gastric cancer organoids were cultured with EGF, FGF10, NOG, RSPO1. (Routinely tested).
11. Human stomach organoids organoids were cultured with EGF, FGF10, NOG, RSPO1. (Routinely tested).
12. Human breast cancer organoids were cultured with FGF7, RSPO1, IGF1, EGF, NRG1 Beta 1, NOG. (Routinely tested).
13. Human ovarian organoids were cultured with IGF1, NRG1 Beta 1,RSPO1, EGF, NOG. (Routinely tested).

説明

R-Spondin 1/RSPO1 Protein, Human, Recombinant is expressed in CHO mammalian cells. The predicted molecular weight is 26.8 kDa and the accession number is Q2MKA7-1.

Species	Human
Expression Host	CHO
Tag	Tag Free
Accession Number	Q2MKA7-1
別名	RSPO, R-spondin 1, CRISTIN3
Construction	A DNA sequence encoding the human RSPO1 (NP_001033722.1) (Met1-Ala263) was expressed and purified.
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC
分子量	26.8 kDa (predicted)

Endotoxin	< 10 EU per mg of the protein.
Formulation	Lyophilized from sterile PBS, PH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.

参考文献

1. Kamata T, et al. (2004) R-spondin, a novel gene with thrombospondin type 1 domain, was expressed in the dorsal neural tube and affected in Wnts mutants. Biochim Biophys Acta. 1676(1):51-62. 2. Ota T, et al. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 36(1):40-5. 3. Strausberg RL, et al. (2003) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):16899-903.

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R-Spondin 1/RSPO1 Protein, Human, Recombinant

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