R-Spondin 2/RSPO2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 48.01 kDa and the accession number is Q6UXX9-1.
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
100 μg | 在庫あり | ¥ 103,500 |
200 μg | 約5 days | ¥ 182,500 |
500 μg | 約5 days | ¥ 385,500 |
生物学的情報 |
Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 2-12 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.
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説明 | R-Spondin 2/RSPO2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 48.01 kDa and the accession number is Q6UXX9-1. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | C-hFc |
Accession Number | Q6UXX9-1 |
別名 | R-spondin 2, CRISTIN2 |
Construction | The Human RSPO2 (Q6UXX9-1, with mutations L186P) (Met1-Gly205) was expressed with the Fc region of Human IgG1 at the C-terminus. |
Protein Purity |
> 90 % as determined by SDS-PAGE
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分子量 | 48.01 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing Histidine, Arginine, 120 mM NaCl, 0.02% Tween 80, 5% sucrose, pH 6. 0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | R-spondin-2, also known as RSPO2, synergizes with Wnt to activate beta-catenin. RSPO2 is secreted proteins that regulate beta-catenin signaling. Activator of the beta-catenin signaling cascade leads to TCF-dependent gene activation. Action both in the canonical Wnt / beta- catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled / LRP receptors. Probably also acts as a ligand for frizzled and LRP receptors. The encoding gene Rspo2 was identified as a novel common integration site for the mouse mammary tumor virus in viral induced mouse mammary tumors. Rspo2 and Rspo2 / Wnt1 tumors contained many spindle cells, consistent with an epithelial-mesenchymal transformation phenotype. When Rspo2 and Rspo2 / Wnt1 tumor cells were transferred into naive mice, they exhibited greater metastatic activity than cells derived from Wnt1 tumors. |
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Please read the User Guide of Recombinant Proteins for more specific information.
R-Spondin 2/RSPO2 Protein, Human, Recombinant (hFc) CRISTIN-2 R-spondin 2 CRISTIN2 CRISTIN 2 recombinant recombinant-proteins proteins protein