RTN4 Protein, Human, Recombinant (GST)

カタログ番号 TMPY-02001

別名: UNG1, NOGO, reticulon 4, RTN4-A, Nbla00271, NOGO-A, RTN-X, NSP, UNG15, ASY, Nbla10545, NI220/250, UDG, DGU, RTN4-B1, RTN4-B2, HIGM4, Nogo-C, UNG2, RTN4-C, DKFZp781L1143, NOGOC, Nogo-B, NSP-CL

Reticulon-4, also known as Foocen, Neurite outgrowth inhibitor, Nogo protein, Neuroendocrine-specific protein, Neuroendocrine-specific protein C homolog, RTN-x, Reticulon-5 and RTN4, is a multi-pass membrane protein that contains one reticulon domain. Isoform 1 of RTN4 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 of RTN4 is widely expressed except for the liver. Isoform 3 of RTN4 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 of RTN4 is testis-specific. Reticulon-4 / RTN4 is a developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Reticulon-4 / RTN4 regulates neurite fasciculation, branching and extension in the developing nervous system. Reticulon-4 / RTN4 is involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. It regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. Isoform 2 of RTN4 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 of RTN4 inhibit BACE1 activity and amyloid precursor protein processing.

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RTN4 Protein, Human, Recombinant (GST)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 138,000

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参考文献

説明

Species	Human
Expression Host	E. coli
Tag	GST
Accession Number	NP_065393.1
別名	UNG1, NOGO, reticulon 4, RTN4-A, Nbla00271, NOGO-A, RTN-X, NSP, UNG15, ASY, Nbla10545, NI220/250, UDG, DGU, RTN4-B1, RTN4-B2, HIGM4, Nogo-C, UNG2, RTN4-C, DKFZp781L1143, NOGOC, Nogo-B, NSP-CL
Construction	The Human RTN4 (NP_065393.1) N-terminal fragment (Met 1-Val 185) was fused with the GST tag at the N-terminus.
Protein Purity	> 70 % as determined by SDS-PAGE
分子量	46.2 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 150 mM NaCl, 1 mM DTT, 0.2 mM GSH, pH 7.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Reticulon-4, also known as Foocen, Neurite outgrowth inhibitor, Nogo protein, Neuroendocrine-specific protein, Neuroendocrine-specific protein C homolog, RTN-x, Reticulon-5 and RTN4, is a multi-pass membrane protein that contains one reticulon domain. Isoform 1 of RTN4 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 of RTN4 is widely expressed except for the liver. Isoform 3 of RTN4 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 of RTN4 is testis-specific. Reticulon-4 / RTN4 is a developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Reticulon-4 / RTN4 regulates neurite fasciculation, branching and extension in the developing nervous system. Reticulon-4 / RTN4 is involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. It regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. Isoform 2 of RTN4 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 of RTN4 inhibit BACE1 activity and amyloid precursor protein processing.

参考文献

1. Yang J., et al., 2000, Cytogenet. Cell Genet. 88:101-102.Prinjha R., et al., 2000, Nature 403: 383-384. 2. Tagami S., et al., 2000, Oncogene 19: 5736-5746. 3. Murayama K.S., et al., 2006, Eur. J. Neurosci. 24: 1237-1244. 4. Daub H., et al., 2008, Mol. Cell 31:438-448.Choudhary C., et al., 2009, Science 325: 834-840.

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Keywords

RTN4 Protein, Human, Recombinant (GST) UNG1 NOGO reticulon 4 RTN4-A Nbla00271 NOGO-A RTN-X UNG-1 NSP UNG15 ASY DKFZp781L-1143 UNG-15 Nbla10545 NI220/250 UDG UNG 1 DGU RTN4-B1 UNG 15 RTN4-B2 HIGM 4 HIGM4 Nogo-C UNG2 DKFZp781L 1143 RTN4-C DKFZp781L1143 NOGOC HIGM-4 Nogo-B NSP-CL UNG-2 UNG 2 recombinant recombinant-proteins proteins protein