S100A1 Protein, Mouse, Recombinant (His)

S100A1 Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 12.5 kDa and the accession number is P56565.

S100A1 Protein, Mouse, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 138,000

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説明	S100A1 Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 12.5 kDa and the accession number is P56565.

Species	Mouse
Expression Host	E. coli
Tag	His
Accession Number	P56565
別名	AI266795, S100a, S100 calcium binding protein A1, S100
Construction	The Mouse S100A1 (P56565) (Gly 2-Ser 94) was expressed, with a polyhistide tag at the N-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	12.5 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	S100A1 is a Ca2+binding protein of the EF-hand type that belongs to the S100 protein family. S100 proteins consisting of at least 19 members exist as dimers in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell-cycle progression and cell differentiation. This protein has been shown to function in the processes including stimulation of Ca2+-induced Ca2+release, inhibition of microtubule assembly, and inhibition of PKC-mediated phosphorylation. Phosphoglucomutase is a target protein whose activity is antagonistically regulated by S100A1, and recently, S100A1 is also identified as a potent molecular chaperone and a new member of the Hsp70/Hsp90 multichaperone complex. S100A1 displays a tissue-specific expression pattern with highest levels in myocardium and is considered to be an important regulator of cardiac contractility. Accordingly, reduced expression or mutations of S100A1 gene have been implicated in cardiomyopathies.

参考文献

1. Remppis, A .et al., 1996, Biochim. Biophs. Acta. 1313: 253-257. 2. Most, P. et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98: 13889-13894 3. Okada, M. et al., 2004, J. Biol. Chem. 279: 4221-4233. 4. Schafer, W.E. et al., 1995, Genomics. 25: 638-643. 5. Landar, A. et al., 1996, Cell. Calcium. 20: 279-285.

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S100A1 Protein, Mouse, Recombinant (His)

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