S100A4 Protein, Mouse, Recombinant (hFc)

S100A4 Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 40 kDa and the accession number is P07091.

S100A4 Protein, Mouse, Recombinant (hFc)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 138,000

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説明	S100A4 Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 40 kDa and the accession number is P07091.

Species	Mouse
Expression Host	HEK293 Cells
Tag	N-hFc
Accession Number	P07091
別名	metastasin, pk9a, 18A2, FSp1, 42a, PeL98, Capl, S100 calcium binding protein A4, Mts1
Construction	The Mouse S100A4 (P07091) (Ala2-Lys101) was expressed with the Fc region of Human IgG1 at the N-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	40 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	S100A4, also known as metastasis-associated protein Mtsl, belongs to the family of small calcium-binding S100 proteins containing two EF-hand calcium-binding motifs. In humans at least 20 S100 family members that are distributed tissue specifically have been identified, and are involved in a number of cellular processes as transducers of calcium signal. S100A4 is a symmetric homodimer, and undergoes a relatively large conformational change upon the typical EF-hand binding calcium, which is necessary for S100A4 to interact with its protein targets and generate biological effects. It can bind the already known targets p53, F-actin, liprin β, myosin heavy chain II, and prevent their phosphorylation and multimerization. It has been demonstrated that S100A4 is directly involved in tumor metastasis including cell motility, invasion, apoptosis, angiogenesis and differentiation, and appears to be a metastasis factor and a molecular marker for clinical prognosis. Multiple alternatively spliced variants encoding the same protein have been identified.

参考文献

1. Ambartsumian N. et al., 1995, Gene. 159: 125-30. 2. Marenholz I. et al., 2004, Biochem Biophys Res Commun. 322: 1111-22. 3. Helfman DM. et al., 2005, Br J Cancer. 92: 1955-8. 4. Saleem M. et al., 2006, Proc Natl Acad Sci. 103: 14825-30. 5. Boye K. et al., 2008, Int J Cancer. 123: 1301-10. 6. Garrett SC. et al., 2006, J Biol Chem. 281: 677-80. 7. Kriajevska M. et al., 2002, J Biol Chem. 277: 5299-335.

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S100A4 Protein, Mouse, Recombinant (hFc)

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