S100A5 Protein, Human, Recombinant (His)

S100A5 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 14.2 kDa and the accession number is B48219.

S100A5 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 138,000

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説明	S100A5 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 14.2 kDa and the accession number is B48219.

Species	Human
Expression Host	E. coli
Tag	His
Accession Number	B48219
別名	S100D, S100 calcium binding protein A5
Construction	The Human S100A5 (P33763) (Met 1-Lys 110) was expressed, with a polyhistide tag at the N-terminus.
Protein Purity	> 92 % as determined by SDS-PAGE
分子量	14.2 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 10% glycerol, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the?neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. Protein S100-A5, also known as Protein S-100D, S100 calcium-binding protein A5, S100A5 and S100D, is a member of the S100 family which contains two EF-hand domains. S100A5 is also a novel member of the EF-hand superfamily of calcium-binding proteins that is poorly characterized at the protein level. It is expressed in very restricted regions of the adult brain. From birth onwards, S100A5 remained a neuronal-specific protein, only located in a subpopulation of neurons in the spiral ganglion.

参考文献

1. Sch?fer,B.W. et al., 2000, J Biol Chem. 275 (39):30623-30. 2. Gebhardt, C. et al., 2006, Biochem Pharmacol. 72 (11):1622-31. 3. Nonaka, D. et al., 2008, J. Cutan. Pathol. 35 (11): 1014-9. 4. Lim, SY. et al., 2008, J Immunol. 181 (8): 5627-36. 5. Heibeck TH. et al., 2009, J. Proteome Res. 8:3852-61.

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S100A5 Protein, Human, Recombinant (His)

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