SARS-CoV-2 Methyltransferase/ME Protein (His)

Coronavirus encodes the 2’-O-MTase (2'O Methyltransferase) that is composed of the catalytic subunit nsp16 and the stimulatory subunit nsp10 and plays an important role in virus genome replication and evasion from innate immunity during viral infection. Nonstructural protein 16 (NSP16) / viral 2'O-methyltransferase (2'O-MTase) is highly conserved. The conserved 2'O-MTase activity is important for CoV pathogenesis and NSP16 is a conserved universal target for rapid live attenuated vaccine design in an expanding Coronavirus outbreak setting, such as COVID-19. Targeting the 2'O-methylation pathway on SARS-CoV replication and pathogenesis can be the treatment options for vaccine and anti-viral drug development which can against SARS-CoV-2，SARS-CoV, MERS-CoV or other RNA and DNA viruses.

SARS-CoV-2 Methyltransferase/ME Protein (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 160,500

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説明

Species	SARS-CoV-2
Expression Host	E. coli
Tag	C-His
Accession Number	YP_009724389.1
Construction	The SARS-CoV-2 (2019-nCoV) Methyltransferase / ME (YP_009724389.1) (Ser6799-Asn7096) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	> 85 % as determined by SDS-PAGE.
分子量	33.46 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Supplied as sterile 10 mM Tris 250 mM NaCl, 50% Glycerol pH 7.4.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	Coronavirus encodes the 2’-O-MTase (2'O Methyltransferase) that is composed of the catalytic subunit nsp16 and the stimulatory subunit nsp10 and plays an important role in virus genome replication and evasion from innate immunity during viral infection. Nonstructural protein 16 (NSP16) / viral 2'O-methyltransferase (2'O-MTase) is highly conserved. The conserved 2'O-MTase activity is important for CoV pathogenesis and NSP16 is a conserved universal target for rapid live attenuated vaccine design in an expanding Coronavirus outbreak setting, such as COVID-19. Targeting the 2'O-methylation pathway on SARS-CoV replication and pathogenesis can be the treatment options for vaccine and anti-viral drug development which can against SARS-CoV-2，SARS-CoV, MERS-CoV or other RNA and DNA viruses.

参考文献

1. Wang Y,et al., Coronavirus nsp10/nsp16 Methyltransferase Can Be Targeted by nsp10-Derived Peptide In Vitro and In Vivo To Reduce Replication and Pathogenesis.J Virol. 2015 2. Yu Chen,et al., Biochemical and Structural Insights into the Mechanisms of SARS Coronavirus RNA Ribose 2′-O-Methylation by nsp16/nsp10 Protein Complex.PLOS Pathogens.2011 3. Mickaël Bouvet,et al.,Coronavirus Nsp10: a Critical Co-Factor for Activation of Multiple Replicative Enzymes.JBC.2014

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SARS-CoV-2 Methyltransferase/ME Protein (His)

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